IL1B Antibody (Biotin)

SKU: orb1671473

Description

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Infectious Disease & Virology, Metabolism Research, Neuroscience

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, WB
Reactivity	Human
Application Notes	Sandwich ELISA: To detect hIL-1 beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Human IL-1 beta as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-1 beta. Western Blot To detect hIL-1 beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1 beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Goat
Clonality	Polyclonal
Immunogen	Produced from sera of Goats pre-immunized with highly pure (>98%) recombinant hIL-1 beta. Anti-HumanIL-1beta specific antibody was purified by affinity chromatography and then biotinylated.
Target	IL1B
Purification	IL-1 beta specific antibody was purified by affinity chromatography and then biotinylated
Conjugation	Biotin

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Concentration	batch dependent
Disclaimer	For research use only

Alternative Names

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Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF)

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To detect hIL-1 beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-1 beta as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-1 beta.

To detect hIL-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Quick Database Links

Gene Symbol

IL1B

UniProt

P01584

RefSeq:P01584

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	P01584

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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