IL-1 beta Recombinant Rabbit Monoclonal Antibody

SKU: orb1610317

Description

Research Area

Cell Biology, Inflammation, Inflammatory Mediators, Interleukins, Obesity, SARS /COVID-19

Images & Validation

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Item 1 of 1

Tested Applications	WB
Dilution Range	WB=1:1000-5000
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	1T3
Immunogen	A synthesized peptide derived from human IL 1 beta (200-269aa)
Target	IL1B
Molecular Weight	31 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 0.05% rAlbumin, 40% Glycerol, 0.02% Proclin300.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IL1B_HUMAN; Interleukin-1 beta; interleukin 1 beta; IL1F2; IL-1 beta; Catabolin; IL-1; IL1F2; IL1beta; IL1-BETA; IL-1beta; IL-1 beta; IL-1β; IL1β; IL-1B; IL1B;

Quality Guarantee

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IL-1 beta Recombinant Rabbit Monoclonal Antibody

Sample: Lane 1: Human THP-1 cell lysates, Lane 2: Human THP-1 treated with 80nM TPA for 24h, then treated with 100 ng/ml lipopolysaccharide (LPS) for 6h, then with 300 ng/ml Brefeldin A added after 3 hours, Primary: Anti-IL-1 beta (orb1610317) at 1/5000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kDa, Observed band size: 35 kDa.

Quick Database Links

Gene Symbol

IL1B

UniProt

P01584

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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