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IL10 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, NeA, WB |
|---|---|
| Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 15B9-1-2 |
| Immunogen | E.coli derived Recombinant Murine IL-10 |
| Target | IL10 |
| Purification | This IgG2A antibody was purified from cell culture by Protein G affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−IL10 antibody [orb221323]
IHC-P
Human, Mouse, Porcine, Rat
Rabbit
Polyclonal
Unconjugated
100 μg, 200 μgIL-10 rabbit pAb Antibody [orb766743]
ELISA, IF, IHC, WB
Human, Mouse, Rat
Polyclonal
Unconjugated
100 μl

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To a sandwich ELISA (assuming 100 ul/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 1600 pg/ml of recombinant murine IL-10 when used with biotinylated antigen affinity purified anti-murine IL-10 (orb1272494) as the detection antibody at a concentration of approximately 0.5-1.0 ug/ml.

To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.

To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_000563.1 |
|---|
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Protocol Information
IL10 Antibody (orb1272609)
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