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IL10 Antibody
SKU: orb1272609
Description
Research Area
Cancer Biology, Infectious Disease & Virology, Neuroscience
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, NeA, WB |
|---|---|
| Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 15B9-1-2 |
| Immunogen | E.coli derived Recombinant Murine IL-10 |
| Target | IL10 |
| Purification | This IgG2A antibody was purified from cell culture by Protein G affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin 10Interleukin-10, Cytokine synthesis inhibitory factor, IL-10, CSIF, TGIF, GVHDS, IL10A
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To a sandwich ELISA (assuming 100 ul/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 1600 pg/ml of recombinant murine IL-10 when used with biotinylated antigen affinity purified anti-murine IL-10 (orb1272494) as the detection antibody at a concentration of approximately 0.5-1.0 ug/ml.
To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000563.1 |
|---|
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL10 Antibody (orb1272609)
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