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Il10 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, NeA, WB |
|---|---|
| Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rIL-10 (rat Interleukin-10). |
| Target | Il10 |
| Purification | Anti-rIL-10 specific antibody was purified by affinity chromatography employing immobilized rIL-10 matrix. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−IL10 antibody [orb221323]
IHC-P
Human, Mouse, Porcine, Rat
Rabbit
Polyclonal
Unconjugated
100 μg, 200 μgIL-10 rabbit pAb Antibody [orb766743]
ELISA, IF, IHC, WB
Human, Mouse, Rat
Polyclonal
Unconjugated
100 μl

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To detect Rat IL-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Rat IL-10 (orb1272492) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-10.

To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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Protocol Information
Il10 Antibody (orb1272493)
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