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IL10 Antibody
SKU: orb750687
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:20,000 - 1:100,000, WB: 1:2,000 - 1:10,000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Antiserum |
| Immunogen | The whole rabbit serum was prepared by repeated immunizations with human IL-10. |
| Target | IL10 |
| Purity | The antiserum detects recombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-10 production from fibroblasts). The antibody recognizes hu IL-10. The antibody will not neutralize the biological activity of murine IL-10. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 85 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−CSIF antibody, Cytokine Synthesis Inhibitory Factor antibody, IL 10 antibody, IL10A antibody, rabbit anti-interleukin 10 antibody, rabbit anti-IL-10, Interleukin10 antibody, MGC126450 antibody, MGC126451 antibody, TGIF antibody
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Biorbyt Anti IL-10 whole antiserum (orb750686) was tested against recombinant IL10. Recombinant IL-10 was run on a 4-20% reducing gel, and transferred to nitrocellulose. Membrane was blocked in 3% BSA-TBS for 1 Hour 4°C and probed with (p/n orb750686) diluted 1:200 in 3% BSA-TBS o/n 4°C. Primary antibody was detected with HRP Gt anti-Rb (orb347654) 1:20000 in orb348637 1 Hour 4°C using FemtoMax Super sensitive chemiluminescent substrate.
Western Blot of Rabbit anti-IL-10 antibody. Lane 1: Recombinant Human IL-10. Load: 50 ng per lane. Primary antibody: IL-10 antibody at 1:500 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (orb348637) for 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for IL-10.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL10 Antibody (orb750687)
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