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Il12a , Il12b Antibody
SKU: orb1671476
Description
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| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rat |
| Clonality | Monoclonal |
| Immunogen | Produced in Sprague Dawley rats immunized with highly pure Recombinant Murine IL-12. Anti-Murine IL-12-specific antibody was purified from cell culture by Protein G affinity chromatography. |
| Target | Il12a , Il12b |
| Purification | IL-12-specific antibody was purified from cell culture by Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF
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In a sandwich ELISA (assuming 100 μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000 pg/ml of Recombinant Murine IL-12 when used with Biotinylated Antigen Affinity Purified anti-Murine IL-12 as the detection antibody at a concentration of approximately 1.0-2.0 μg/ml.
To detect Murine IL-12 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-12 is 1.0-2.0 ng/lane, under reducing conditions and 0.25-0.5 ng/lane, under non-reducing conditions.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Il12a , Il12b Antibody (orb1671476)
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