IL-12 Recombinant Protein

SKU: orb1230693

Description

Images & Validation

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Key Properties

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Source	CHO cells
Biological Activity	Determined by its ability to induce cell proliferation in T-cell enriched PBMCs. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
Target	Il12a
Protein Sequence	p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS
Purity	Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Storage & Handling

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Storage	The lyophilized IL-12 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted IL-12 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing IL-12 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Form/Appearance	Lyophilized
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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p35, Ll12a, Il-12a, IL-12p35, Interleukin-12 subunit alpha, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12A

Recombinant Human IL-23 Protein [orb2993911]

Conjugation:	Unconjugated
Purity:	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 90% as determined by SEC-HPLC. (QC verified)
MW:	Predicted: 19.7&34.8 KDa. Observed: 19-21&40-60 KDa, reducing conditions

Sizes Available: 10 μg, 50 μg, 500 μg, 1 mg

Recombinant Human IL-23 Protein [orb2993914]
Conjugation:
Unconjugated
Purity:
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
MW:
Predicted: 81.3 KDa. Observed: 80-110 KDa, reducing conditions
Sizes Available: 10 μg, 50 μg, 1 mg, 500 μg
Recombinant Mouse IL-23 Protein [orb2993830]
Conjugation:
Unconjugated
Purity:
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
MW:
Predicted: 19.7&35.8 KDa. Observed: 40&20 KDa, reducing conditions
Sizes Available: 1 mg, 10 μg, 50 μg, 500 μg

Quality Guarantee

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Quick Database Links

Gene Symbol

Il12a

UniProt

P43431

RefSeq (Protein):NP_032377.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_032377.1

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