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IL-15 Recombinant Protein
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Target | Il15 |
| Tag | Fc Tag |
| Molecular Weight | 39.7 kDa |
| Protein Sequence | Asn 49 - Ser 162 |
| Purity | >95% as determined by SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human IL15 Protein, hFc Tag [orb1291067]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 38.9 kDa after removal of the signal peptide. The apparent molecular mass of IL15-hFc is approximately 35-55kDa due to glycosylation.
Mammalian
50 μg, 10 μg, 100 μgRecombinant Cyn. monkey CD122 / IL2RB protein, C-His (HEK293) [orb1516513]
>95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 43-47 kDa based on Tris-Bis PAGE result.
100 μg, 50 μgHuman IL-15RA&IL-15 [orb3002349]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 34.4&12.8 KDa. Observed: 37&17 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman IL-15RA&IL-15 [orb3002427]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 46.9 KDa. Observed: 50-60 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgIL-15 R alpha / CD215 Recombinant Protein [orb1231304]
ELISA, WB
>95% as determined by SDS-PAGE.
44.8 kDa
HEK293 cells
0.1 mg

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Mouse IL-15, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Immobilized Mouse IL-15, Fc Tag at 2 ug/ml (100 uL/well) can bind Biotinylated Human IL-15 R alpha, Fc, Avitag with a linear range of 10-156 ng/mL.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_032383.1 |
|---|
Documents Download
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Protocol Information
IL-15 Recombinant Protein (orb1231338)
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