IL17A Antibody (Biotin)

SKU: orb345174

This product can be custom made

Description

IL-17A antibody (Biotin)

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
Reactivity	Human
Application Notes	This purified antibody has been tested in western blotting. By western blot a band approximately 30.3 kDa in size corresponding to human IL17-A protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL17-A protein.
Target	IL17A
Purity	This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL17-A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Conjugation	Biotin

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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rabbit anti-Interleukin-17A biotin conjugated antibody, rabbit anti-IL-17A biotin conjugated antibody, IL-17A, Interleukin-17A, Cytotoxic T-Lymphocyte-associated Antigen 8, CTLA8, Interleukin-17, IL17, IL-17, Interleukin17

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Western blot using Biorbyt's anti-Human IL17-A antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG secondary antibody diluted 1:20000 in blocking buffer (p/n orb348637).

Quick Database Links

Gene Symbol

IL17A

UniProt

Q16552

RefSeq:AAH662531.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	AAH662531.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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