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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, NeA, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | E.coli derived Recombinant Human IL-1Ra |
| Target | IL1RN |
| Purification | IL-1RA specific antibody was purified by affinity chromatography employing an immobilized Human IL-1RA matrix. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−DIRA, IRAP, IL1F3, IL1RA, MVCD4, IL-1RN, IL-1ra, IL-1ra3, ICIL-1RA, Interleukin-1 receptor antagonist protein
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To detect Human IL-1RA by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-1RA (orb1272756) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-1RA.
To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-1RA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-1RA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL1RN Antibody (orb1272616)
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