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IL-21 Antibody
SKU: orb344651
Description
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, WB |
|---|---|
| Dilution range | ELISA: 1:6,000, WB: 1 µg/mL |
| Reactivity | Bovine, Equine, Porcine |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to amino acid residues 24-152 of bovine IL-21 protein. |
| Purity | This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for bovine IL-21 protein. A BLAST analysis was used to suggest cross-reactivity with IL-21 from bovine sources based on 100% homology with the immunizing sequence. Based on 92% homology, there is a chance of cross-reactivity to porcine IL- 21, 91% to horse IL-21, 89% to human and dog, 88% to macaque, 84% to platypus and Syrian hamster, 76-79% to mouse, 75% to rat, and 48% to chicken. Cross-reactivity with IL-21 from other sources has not been determined. |
Storage & Handling
−| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-IL21 Antibody, Interleukin-21, IL-21, Interleukin21, Interleukin 21, IL 21
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Western blot using Biorbyt's anti-bovine IL-21 antibody shows detection of recombinant bovine IL-21 at 15.1kDa (arrow) raised in yeast. Protein was purified and resolved by SDS-PAGE, transferred to PVDF membrane. Membrane was blocked with 3% BSA (diluted 1:10), and probed with Biorbyt's Anti-bovine IL-21. After washing, membrane was probed with Dylight ™ 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL-21 Antibody (orb344651)
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