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IL-22 Antibody
SKU: orb345155
Description
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, WB |
|---|---|
| Dilution range | ELISA: 1:20,000 - 1:50,000, WB: 1:1000-1:2000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-IL-22 antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human IL-22 protein from E. coli. |
| Purity | Anti-IL-22 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-22 protein. A BLAST analysis was used to suggest cross-reactivity with IL-22 from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-22 from other sources has not been determined. |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-IL-22 antibody, rabbit anti-interleukin-22 antibody, Interleukin22, IL22, ILTIF, ZCYTO18, IL22, Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor
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Western Blot of Rabbit anti-IL-22 antibody. Lane 1: Human IL-22 Recombinant Protein. Load: 50 ng per lane. Primary antibody: IL-22 antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 14 kDa, 14 kDa for Human IL-22. Other band(s): None.
Quick Database Links
UniProt
RefSeq (Protein):NP_065386.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_065386.1 |
|---|
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL-22 Antibody (orb345155)
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