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IL-22 Antibody
SKU: orb345156
Description
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, WB |
|---|---|
| Dilution range | ELISA: 1:20,000 - 1:50,000, WB: 1:1000-1:2000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-IL-22 antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human IL-22 protein from E. coli. |
| Purity | Anti-IL-22 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-22 protein. A BLAST analysis was used to suggest cross-reactivity with IL-22 from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-22 from other sources has not been determined. |
Storage & Handling
−| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-IL-22 antibody, rabbit anti-interleukin-22 antibody, Interleukin22, IL22, ILTIF, ZCYTO18, IL22, Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor
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Western Blot of Rabbit anti-IL-22 antibody. Lane 1: Human IL-22 Recombinant Protein. Load: 50 ng per lane. Primary antibody: IL-22 antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 14 kDa, 14 kDa for Human IL-22. Other band(s): None.
Quick Database Links
UniProt
RefSeq (Protein):NP_065386.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_065386.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL-22 Antibody (orb345156)
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