IL-4 Antibody Peroxidase Conjugated

SKU: orb345166

Description

IL4 antibody (Peroxidase)

Images & Validation

−

Item 1 of 1

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:10,000-1:50,000, IHC: 1:500;1:2,500, WB: 1:1,000-1:5,000
Reactivity	Human
Application Notes	This purified antibody has been tested in western blotting and suitable for ELISA. By western blot a band approximately 15 kDa in size corresponding to human IL-4 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-4 protein.
Purity	This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-4 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Conjugation	HRP

Storage & Handling

−

Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

−

rabbit anti-IL-4 peroxidase conjugated antibody, rabbit anti-interleukin-4 peroxidase conjugated antibody, BCGF-1, BCGF1, Binetrakin, BSF 1, BSF1, HCGF, IA Inducing Factor, IGG1 induction factor, IL 4, Il4e12, Interleukin 4, Interleukin 4, isoform 1, Interleukin4, Lymphocyte stimulatory factor 1, MCGF2, MFF, Pitrakinra, TCGF2

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Western Blot of Rabbit Anti-IL4 Antibody Peroxidase Conjugated. Lane 1: Human IL-4. Load: 50 ng per lane. Secondary antibody: IL-4 Antibody Peroxidase Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 30 min RT. Predicted/Observed size: 18 kDa, 18 kDa.

Quick Database Links

UniProt

P05112

RefSeq (Protein):NP_000580.1

UniProt Details

−

No UniProt data available

NCBI Reference Sequences

−

Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000580.1

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols