IL-5 Recombinant Protein

SKU: orb1231321

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Description

Research Area

Cancer Biology, Immunology & Inflammation, Metabolism Research, Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, WB
Application Notes	This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 15.0 kDa. The protein migrates as 18-23 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Source	HEK293 cells
Target	IL5
Tag	His Tag
Molecular Weight	15.0 kDa
Protein Sequence	Ile 20 - Ser 134
Purity	>90% as determined by SDS-PAGE.

Storage & Handling

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Storage	Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	PBS, pH 7.4
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IL-5,TRF,IL5,Interleukin-5

Human IL5 Protein, His Tag [orb689428]

Conjugation:	Unconjugated
Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 13.94 kDa after removal of the signal peptide.The apparent molecular mass of IL5-His is approximately 15-25 kDa due to glycosylation.
Source:	Mammalian

Sizes Available: 10 μg, 100 μg, 50 μg

Recombinant Mouse IL-5RA Protein [orb2994722]
Conjugation:
Unconjugated
Purity:
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
MW:
Predicted: 37.6 KDa. Observed: 48 KDa, reducing conditions
Sizes Available: 10 μg, 50 μg, 500 μg, 1 mg
Recombinant Human IL-5RA Protein [orb2994170]
Conjugation:
Unconjugated
Purity:
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
MW:
Predicted: 36.7 KDa. Observed: 45-60 KDa, reducing conditions
Sizes Available: 10 μg, 50 μg, 500 μg, 1 mg
Recombinant Human IL-5RA Protein [orb2994171]
Conjugation:
Unconjugated
Purity:
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
MW:
Predicted: 62.5 KDa. Observed: 70-85 KDa, reducing conditions
Sizes Available: 10 μg, 50 μg, 500 μg, 1 mg

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Human IL-5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Immobilized Human IL-5, His Tag at 5 ug/ml (100 uL/well) can bind Human IL-5 R alpha, Fc Tag with a linear range of 0.02-0.313 ug/ml.

Quick Database Links

Gene Symbol

IL5

UniProt

P05113

RefSeq (Protein):NP_000870.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000870.1

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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