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Il6 Antibody
SKU: orb1272443
Description
Research Area
Immunology & Inflammation, Neuroscience
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, NeA, WB |
|---|---|
| Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Rat IL-6. |
| Target | Il6 |
| Purification | Anti-Rat IL-6 specific antibody was purified by affinity chromatography employing immobilized Rat IL-6 matrix. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−ILg6, Ifnb2, Il-6, Interleukin-6IL-6
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To detect Rat IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Rat IL-6 (orb1272442) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-6.
To detect Rat IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained colchicine injected rat brain (cingulate cortex) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Il6 Antibody (orb1272443)
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