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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 2
| Tested Applications | DOT, ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:5,000-1:50,000, WB: 1:1,000 - 1:10,000 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of mouse IL-7 receptor protein. |
| Target | IL17R |
| Purity | This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using peptide coupled to agarose beads. This antibody is specific for mouse IL-7 receptor protein. A BLAST analysis was used to suggest cross-reactivity with IL-7 receptor protein from human, mouse and rat sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-7 receptor protein from other sources has not been determined. Anti-IL-7 receptor alpha chain antibody is useful for researchers interested in Immunology Research. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store IL-7 Receptor Alpha Chain Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.15 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-IL-7 Receptor alpha chain antibody, rabbit anti-Interleukin-7 Receptor alpha chain antibody, IL7R alpha antibody, IL-7R alpha, IL 7R α, Interleukin7 Receptor, Interleukin 7 receptor alpha chain antibody, Interleukin 7 receptor α antibody
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ELISA results of purified Rabbit anti-IL 7 Receptor Alpha Chain Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1 µg of conjugate. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n orb347654) and TMB ELISA Peroxidase Substrate (p/n orb348651).
Western Blot of Rabbit Anti-IL-7 Receptor alpha chain Antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: Human Spleen Whole Cell Lysate [30 µl] (+). Lane 3: U251 Whole Cell Lysate (p/n orb692720) [30 µl] (+). Lane 4: Human Pancreas Whole Cell Lysate [30 µl] (-). Lane 5: MOLT-4 Whole Cell Lysate (p/n orb348696) [30 µl] (+). Primary Antibody: Anti-IL-7R at 1.0 µg/ml overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG Peroxidase (p/n orb347654) at 1:70000 for 30 mins at RT. Block: BlockOut Buffer (p/n orb348644) for 1hr at RT. Exposure: 5 seconds. Predicted MW: ~55kDa.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DOT
Dot Blot
IL17R Antibody (orb345607)
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