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IL17RA Antibody
SKU: orb1238913
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | IL-17RA antibody was raised against an 18 amino acid peptide near the amino terminus of human IL-17RA.The immunogen is located within amino acids 150 - 200 of IL-17RA. |
| Target | IL17RA |
| Molecular Weight | Predicted: 95 kDa Observed: 135 kDa |
| Purification | IL-17RA Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | IL-17RA Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−IL-17RA Antibody: CD217, IL17R, CANDF5, CDw217, IL-17RA, hIL-17R, Interleukin-17 receptor A, IL-17 receptor A
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WB Validation in K562 Cells. Loading: 10 μg of lysate. Antibodies: IL-17RA, orb1238913, 4 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
WB Validation in Mouse Tissues. Loading: 15 μg of lysate. Antibodies: IL-17RA, orb1238913, 2 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of IL-17RA in Human Spleen. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling IL-17RA with orb1238913 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Immunofluorescence Validation of IL-17RA in Mouse Thymus. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus tissue labeling IL-17RA with orb1238913 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL17RA Antibody (orb1238913)
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