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IL9 Antibody
SKU: orb1239508
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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-IL-9 antibody (orb1239508) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IL-9. The immunogen is located within the last 50 amino acids of IL-9. |
| Target | IL9 |
| Molecular Weight | Predicted: 16kDObserved: 22 kD (Post-modification: 4 N-linked glycosylations) |
| Purification | IL-9 Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | IL-9 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−IL-9 Antibody : P40, HP40, IL-9, Interleukin-9, Cytokine P40
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Western Blot Validation in Human Spleen Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: IL-9, orb1239508 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IL-9 recombinant protein per lane. Antibodies: IL-9, orb1239508 (Lane 1: 1 µg/mL, Lane 2: 2 µg/mL and Lane 3: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 22kD.
Western Blot Validation in Tissue and Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: IL-9, orb1239508 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of IL-9 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-9 antibody (orb1239508) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of IL-9 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-9 antibody (orb1239508) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL9 Antibody (orb1239508)
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