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IL1RN Antibody / Interleukin-1 Receptor Antagonist / IL-1RA
SKU: orb1823877
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 2-4ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2, kappa |
| Clone No. | IL1RA/4716 |
| Immunogen | A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the IL1RN antibody. |
| Purification | Protein A/G affinity |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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IL1RN Antibody / Interleukin-1 Receptor Antagonist / IL-1RA [orb1823876]
IHC-P, WB
Human
Mouse
Monoclonal
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100 μg
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IHC staining of FFPE human tonsil tissue with IL1RN antibody (clone IL1RA/4716). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human adrenal gland tissue with IL1RN antibody (clone IL1RA/4716). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
Analysis of a HuProt (TM) microarray containing more than 19000 full-length human proteins using IL1RN antibody (clone IL1RA/4716). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.
SDS-PAGE analysis of purified, BSA-free IL1RN antibody (clone IL1RA/4716) as confirmation of integrity and purity.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IL1RN Antibody / Interleukin-1 Receptor Antagonist / IL-1RA (orb1823877)
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