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IL1RN Antibody (C-term)
SKU: orb1930039
Description
Research Area
Cardiovascular Research, Immunology & Inflammation, Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IF, WB |
|---|---|
| Dilution range | WB - 1:1000, FC - 1:10-50, IF - 1:10-50 |
| Reactivity | Human, Mouse |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 20055 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
−IL1F3, IL1RA
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IL1RN Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of IL1RN Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
IL1RN Antibody (C-term) western blot analysis in 293, Hela cell line lysates (35 ug/lane). This demonstrates the IL1RN antibody detected the IL1RN protein (arrow).
Anti-IL1RN Antibody (C-term) at 1:4000 dilution + 293 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 20 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
IL1RN Antibody (C-term) (orb1930039)
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