Recombinant IL3RA Antibody / CD123

SKU: orb2642614

Description

CD123/IL3RA is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3.

Images & Validation

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Tested Applications	IHC-P
Dilution range	Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Reactivity	Human
Application Notes	The stated application concentrations are suggested starting points. Titration of the recombinant IL3RA antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	CDLA123-1R
Immunogen	A portion of amino acids 26-171 was used as the immunogen for the recombinant IL3RA antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC staining of FFPE human tonsil with IL3RA antibody (clone CDLA123-1R). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min and allow to cool.

SDS-PAGE analysis of purified, BSA-free IL3RA antibody (clone CDLA123-1R) as confirmation of integrity and purity.

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UniProt

P26951

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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