IL6/Interleukin 6 Antibody

SKU: orb1536892

Description

Images & Validation

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Tested Applications	ELISA, IHC, IHC-P, WB
Dilution range	ELISA (1:10000), IHC, IHC-P (1:200), WB (1:100 - 1:500)
Reactivity	Human
Application Notes	Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 1:200. Read more...

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	6D9A1
Immunogen	Purified recombinant fragment of human IL-6 expressed in E. coli.
Target	IL6 / Interleukin 6
Purification	Ascites
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Ascitic fluid, 0.03% sodium azide
Disclaimer	For research use only

Alternative Names

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IL6, B-cell stimulatory factor 2, BSF-2, BSF2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, Interleukin-6, Interleukin BSF-2, IFNB2, B-cell differentiation factor, CDF, HSF, IL-6

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Gene Symbol

IL6 / Interleukin 6

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Datasheet

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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