IL6/Interleukin 6 Antibody

SKU: orb1539004

Description

Images & Validation

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Tested Applications	ELISA, IHC-P, WB
Dilution range	ELISA (0.5 - 2 µg/ml), IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml)
Reactivity	Human
Application Notes	Resuspension: Reconstitute in sterile water to 0.1-1 mg/ml. . Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-6 (1 ng/ml), a concentration of 0.20-0.40 ug/ml of this antibody is required. ELISA: To detect Human IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-6 as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-6. Western Blot: To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-6 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. Read more...

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	E.coli derived recombinant Human IL-6.
Target	IL6 / Interleukin 6
Purification	Immunoaffinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Lyophilized from PBS, pH 7.2
Disclaimer	For research use only

Alternative Names

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IL6, B-cell stimulatory factor 2, BSF-2, BSF2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, Interleukin-6, Interleukin BSF-2, IFNB2, B-cell differentiation factor, CDF, HSF, IL-6

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

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Gene Symbol

IL6 / Interleukin 6

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Datasheet

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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