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Indomethacin

SKU: orb1310593

Description

Indomethacin

Research Area

Cell Biology, Immunology & Inflammation, Metabolism Research, Neuroscience, Pharmacology & Drug Discovery

Images & Validation

Key Properties

CAS Number53-86-1
MW357.79
Purity99.27% (May vary between batches)
FormulaC19H16ClNO4
SMILESCOC1=CC2=C(C=C1)N(C(=O)C1=CC=C(Cl)C=C1)C(C)=C2CC(O)=O
TargetPhospholipase,COX,PDE,Autophagy
SolubilityEthanol:17.9 mg/mL (50.03 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.58 mg/mL (10.01 mM);DMSO:104 mg/mL (290.67 mM)

Bioactivity

Target IC50
COX-1:0.28 μM|COX-2:14 μM
In Vivo
METHODS: To test the antitumor activity in vivo, Indomethacin (1 mg/kg) was administered by gavage to CDF1 mice bearing Colon 26 xenografts twice daily for 7-21 days. RESULTS: Mean tumor weight was significantly lower in both the long-term and short-term Indomethacin administration groups, serum immunosuppressive acidic protein levels were significantly lower, serum IL-6 levels were also significantly lower, and prostaglandin E2 levels in tumor tissues were significantly lower than those in the control group.
In Vitro
METHODS: Human gastric cancer cells AGS were treated with Indomethacin (100-500 µM) for 24 h, and the expression levels of target proteins were detected by Western Blot. RESULTS: Indomethacin increased LC3-II protein levels in AGS cells, which may be a result of induction of autophagy or inhibition of lysosome-dependent autophagic degradation. METHODS: Human tumor cells HT29 and A431 were treated with Indomethacin (1-10 µM) and EGF (25 ng/mL) for 24-48 h. Cell migration was detected by wound-healing assay. RESULTS: Indomethacin inhibited HT29 cell migration. After treating A431 cells with EGF only, the wound closed within 24 h, but the wound remained open after 24 h of treatment with Indomethacin.

Storage & Handling

Storage-20°C
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

COX-2, COX-1, COX, Cyclooxygenase, anticancer, anti-infective, Autophagy, Inhibitor, PDE2, inhibit, Indocin, inflammation, Indometacin, Indometacine, Indomethacin, Indomethacine, PLA2

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Key Properties

No computed properties available.

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Indomethacin (orb1310593)

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Available Sizes

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1 ml x 10 mM (in DMSO)
¥ 910.00
500 mg
¥ 1,040.00
1 g
¥ 1,170.00
5 g
¥ 1,690.00
10 g
¥ 2,210.00
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