Integrin alpha 5 Rabbit mAb [KD Validated]

SKU: orb1951313

Description

Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1.

Images & Validation

−

Item 1 of 3

Tested Applications	FC, ICC, IHC, IP, WB
Dilution range	WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
Reactivity	Human, Mouse, Rat

Key Properties

−

Antibody Type	Recombinant Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG
Immunogen	A synthesized peptide derived from human Integrin alpha 5
Purification	Affinity-chromatography

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Disclaimer	For research use only

Alternative Names

−

Integrin alpha-5; VLA-5; CD49e; ITGA5; FNRA;

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Integrin alpha 5 Rabbit mAb [KD Validated]

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunofluorescent analysis of MCF-7 cells, using Integrin alpha 5 Antibody.

Western blot analysis of Integrin alpha 5 expression in Mouse heart lysate.

Quick Database Links

UniProt

P08648

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

Flow Cytometry

View Protocol

ICC

Immunocytochemistry

View Protocol

Immunoprecipitation

View Protocol

Find More Protocols