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LAG3 Antibody
SKU: orb1239547
Featured
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 8
| Tested Applications | ELISA, FC, ICC, IF, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1,k |
| Clone No. | 2G8 |
| Immunogen | LAG-3 antibody was raised against the extracellular domain of human LAG-3 |
| Target | LAG3 |
| Molecular Weight | Predicted: 55 kDa Observed: 60, 70, 80 kDa |
| Purification | LAG-3 Antibody is supplied as protein A purified IgG1. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | LAG-3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−LAG3 Antibody: lymphocyte activating 3, LAG-3, CD223
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Western blot analysis of LAG-3 in over expressing HEK293 cells using LAG-3 antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml.
Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.
Immunofluorescence of LAG-3 in over expressing HEK293 cells using LAG-3 Antibody at 2 μg/ml. Green: LAG3 Antibody [2G8] (orb1239547) Blue: DAPI staining
Immunofluorescence of LAG-3 in over human spleen tissue using LAG-3 Antibody at 10 μg/ml. Red: LAG3 Antibody [2G8] (orb1239547) Blue: DAPI staining
Immunohistochemistry of LAG-3 in human lymphoma tissue using LAG-3 Antibody and control mouse IgG (corner box) at 5 μg/ml.
Flow cytometry analysis of LAG-3 over expressing HEK293 cells using LAG-3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.
Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA with orb1239547, orb1239545, orb1239551, orb1239549, orb1239550, orb1239548, and orb1239546 antibodies at decreasing concentrations.
A sandwich ELISA was performed using the anti-LAG3 mAbs orb1239547, orb1239545, orb1239551, orb1239549, orb1239550 and orb1239548 as the capture antibodies for the LAG3 extracellular domain antigen with biotin-labeled Risk-Free anti-LAG3 mAbs as the detection antibodies.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
LAG3 Antibody (orb1239547)
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