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LIF Recombinant Protein
SKU: orb1230133
Description
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Maintains pluripotency of mouse and human embryonic stem cells. The LIF (GST-tagged) from AdipoGen has the same activity as cleaved LIF. |
| Target | LIF |
| Tag | GST tag |
| Protein Sequence | Human LIF (aa 23-202) is fused at the N-terminus to a tag. |
| Purity | >95% (SDS-PAGE). Endotoxin level is less than 0.05EU/ μg purified protein (LAL test; Lonza). |
Storage & Handling
−| Storage | Stable for at least 6 months after receipt when stored at -20°C. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Contains PBS. Reconstitute with 100 μl sterile water. |
| Concentration | 0.1mg/ml after reconstitution |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Leukemia Inhibitory Factor, Differentiation-stimulating Factor, D Factor, Melanoma-derived LPL Inhibitor
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 46 kDa after removal of the signal peptide. The apparent molecular mass of LIF-His is approximately 55-70 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg
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LIF Recombinant Protein (orb1230133)
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