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Ccl2 Antibody (Biotin)
SKU: orb1272401
Description
Images & Validation
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| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat MCP-1(MCAF) (rat MCP-1(MCAF)). |
| Target | Ccl2 |
| Purification | Anti-rat MCP-1(MCAF) specific antibody was purified by affinity chromatography and then biotinylated. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | MCP-1/MCAF antibody is stable for at least 2 years from date of receipt at -20˚°C. The reconstituted antibody is stable for at least two weeks at 2-8˚°C. Frozen aliquots are stable for at least 6 months when stored at -20˚°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−MCP-1, Scya2, Sigje, Je, Mcp1, C-C motif chemokine 2, Immediate-early serum-responsive protein JE, MCP-1
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To detect Rat MCP-1 (MCAF) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Rat MCP-1 (MCAF) (orb1272402) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat MCP-1 (MCAF).
To detect Rat MCP-1 (MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1 (MCAF) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat MCP-1 (MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1 (MCAF) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Ccl2 Antibody (Biotin) (orb1272401)
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