Me2-H4(K20) Antibody

SKU: orb1931072

This product can be custom made

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Metabolism Research

Images & Validation

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Item 1 of 4

Tested Applications	DOT, FC, IHC-P, WB
Dilution Range	WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50, DB - 1:500
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	BTD39337
Immunogen	This H4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human H4. Antigen Region: 1-30 aa.
Target	H4C1
Molecular Weight	11367 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Histone H4, HIST1H4A, H4/A, H4FA

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

H4 Antibody (K20 [Me2]) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PSMA4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Dot blot analysis of anti-hH4-K20 (Methyl 2) methylation-specific Pab on nitrocellulose membrane. 50ng of methylation-peptide or Non methylation-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Western blot analysis of lysate from K562 cell line, using H4 Antibody (K20). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug per lane.

Quick Database Links

Gene Symbol

H4C1

UniProt

P62805

RefSeq (Protein):NP_003533.1, NP_778224.1, NP_001029249.1, NP_003529.1, NP_003537.1, NP_003486.1, NP_003539.1, NP_068803.1, NP_003531.1, NP_003536.1, NP_003532.1, NP_003535.1, NP_003530.1, NP_003534.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_003533.1, NP_778224.1, NP_001029249.1, NP_003529.1, NP_003537.1, NP_003486.1, NP_003539.1, NP_068803.1, NP_003531.1, NP_003536.1, NP_003532.1, NP_003535.1, NP_003530.1, NP_003534.1