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CCL28 Antibody
SKU: orb1272639
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits immunized with highly pure recombinant Human MEC. Human MEC specific antibody was purified by affinity chromatography employing an immobilized Human MEC matrix. |
| Target | CCL28 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−MEC, CCK1, SCYA28, C-C motif chemokine 28, Mucosae-associated epithelial chemokine, MEC
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To detect hMEC by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MEC (orb1272628) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMEC.
To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMEC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hMEC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMEC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained CACO-2 cells. The primary antibody was incubated at 3.0 ug/ml overnight at 4 °C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CCL28 Antibody (orb1272639)
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