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Metallothionein Antibody (HRP)

SKU: orb55589

Description

Rabbit polyclonal antibody against Metallothionein-1 Conjugated to HRP

Research Area

Epigenetics & Chromatin, Signal Transduction

Images & Validation

Tested ApplicationsWB
Dilution RangeWB (1:1000)
ReactivityBacteria
Application Notes
A 1:1000 dilution was sufficient for detection of Metallothionein in 15 µg of pseudomonas aeruginosa purified Metallothionein (pmtA-GST) protein by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

HostRabbit
ClonalityPolyclonal
ImmunogenSynthetic peptide from the mid-protein of Pseudomonas aeruginosa Metallothionein
TargetMetallothionein
Molecular Weight36 kDa
PurificationPeptide Affinity Purified
ConjugationHRP

Storage & Handling

StorageConjugated antibodies should be stored according to the product label
Buffer/Preservatives73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Metallothionein, Metallothionein 1A, Metallothionein 1S, Metallothionein IA, Metallothionein II, Metallothionein-1A, Metallothionein-IA, Metallothionein2, Metallothionein 2, Metallothionein 2A, MT1, MT1A, MT1S, MT2, MT2A, MT 1A, MT IA, MT II, MT-1A, MT-IA, MT1A_HUMAN, MGC32848, MTC, pmtA, Probable Metallothionein, CES 1, CES1

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Metallothionein Antibody (HRP)

Western blot analysis of Pseudomonas aeruginosa Purified protein showing detection of ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST) Metallothionein protein using Rabbit Anti-Metallothionein Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Blank. Lane 3: Pseudomonas aeruginosa purified protein with GST tag. Load: 25 μg. Block: 1% milk, 2% BSA. Primary Antibody: Rabbit Anti-Metallothionein Polyclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Mouse Anti-Rabbit IgG: AP at 1:1000 for 60 min at RT. Color Development: Alkaline phosphate activity colorimetric assay. Predicted/Observed Size: ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST).

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol

Metallothionein Antibody (HRP) (orb55589)

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100 μg
¥ 8,320.00
DispatchUsually dispatched within 2-3 weeks
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