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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 3
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | IF - 1:100, WB - 1:1000 |
| Reactivity | Human, Mouse |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | B6CV469 |
| Immunogen | This monoclonal antibody is generated from mice immunized with purified recombinant protein encoding the catalytic domain of human Met. |
| Target | MET |
| Molecular Weight | 155541 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met, MET
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Detection of endogenous Met in HepG2 cell line. 10 μg/lane of HepG2 cell lysate was used to examine the expression of human Met. Lanes 1-5 represent Abgent's different anti-Met monoclonal antibodies. Lane 6 represents auto-phosohorylated-Met in HepG2 cell line detected by anti-phospho-Met Mab.
Fluorescent confocal image of HepG2 cells stained with MET/HGFR Antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MET/HGFR primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).
Western blot analysis of lysates from HepG2, H. liver, HT-29 cell line (from left to right), using MET/HGFR Antibody, which was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary Antibody. Lysates at 35 μg per lane.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001120972.1, NP_000236.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
MET/HGFR Antibody (orb1939454)
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