MET/HGFR Antibody

SKU: orb1939458

This product can be custom made

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Cell Biology

Images & Validation

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Item 1 of 4

Tested Applications	IF, IHC-P, WB
Dilution Range	IHC-P - 1:50-100, WB - 1:8000, IF - 1:100
Reactivity	Human, Mouse

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1
Clone No.	B6CV66
Immunogen	This monoclonal antibody is generated from mice immunized with purified recombinant protein encoding the catalytic domain of human Met. Antigen Region: Unknown.
Target	MET
Molecular Weight	155541 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met, MET

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

All lanes: Anti-MET/HGFR Antibody at dilution. Lane 1: HeLa whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: COS-7 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 156 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of HepG2 cells stained with MET/HGFR Antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MET/HGFR primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MET/HGFR Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunohistochemical analysis of paraffin-embedded H.liver section using MET/HGFR Antibody. diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Quick Database Links

Gene Symbol

MET

UniProt

P08581

RefSeq (Protein):NP_001120972.1, NP_000236.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001120972.1, NP_000236.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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