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CCL4 Antibody (Biotin)
SKU: orb1272370
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hMIP-1-β (human MIP-1-β). |
| Target | CCL4 |
| Purification | Anti-hMIP-1-β specific antibody was purified by affinity chromatography and then biotinylated. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | MIP-1B antibody is stable for at least 2 years from date of receipt at -20˚°C. The reconstituted antibody is stable for at least two weeks at 2-8˚°C. Frozen aliquots are stable for at least 6 months when stored at -20˚°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−ACT2, G-26, HC21, LAG1, LAG-1, MIP1B, SCYA2, SCYA4, MIP1B1, AT744.1, MIP-1-beta, C-C motif chemokine 4, G-26 T-lymphocyte-secreted protein
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CCL4 Rabbit Polyclonal Antibody (Biotin) [orb447596]
IF, IHC-Fr, IHC-P
Human, Mouse, Rat
Human, Mouse, Rat
Rabbit
Polyclonal
Biotin
100 μl
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To detect Human MIP-1-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human MIP-1-beta (orb1272371) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human MIP-1-beta.
To detect Human MIP-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-1-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human MIP-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-1-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CCL4 Antibody (Biotin) (orb1272370)
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