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Ccl4 Antibody (Biotin)

SKU: orb1272749

Description

Ccl4 Antibody (Biotin)

Images & Validation

Tested ApplicationsELISA, WB
ReactivityMouse
Application Notes
ELISA:Sandwich:To detect mMIP-1b by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Murine MIP-1b as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1b. Western Blot:To detect mMIP-1b by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1b is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
ImmunogenProduced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMIP-1β. Murine MIP-1β specific antibody was purified by affinity chromatography and then biotinylated.
TargetCcl4
ConjugationBiotin

Storage & Handling

StorageMIP-1B antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Form/AppearanceLyophilized
Concentrationbatch dependent
DisclaimerFor research use only

Alternative Names

Act-2, Mip1b, Scya4, MIP-1B, AT744.1, C-C motif chemokine 4, Immune activation protein 2, ACT-2

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Ccl4 Antibody (Biotin)

To detect Murine MIP-1-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine MIP-1-beta (orb1272750) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine MIP-1-beta.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP14097

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Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Ccl4 Antibody (Biotin) (orb1272749)

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Available Sizes

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0.05 mg
¥ 10,140.00
DispatchUsually dispatched within 5-10 working days
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