Mouse CD274 protein

SKU: orb391395

Description

Recombinant mouse CD274 protein is produced by our mammalian expression system and the target gene encoding Phe19-Thr238 is expressed with a Fc tag at the C-terminus.

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	Human cells
Reactivity	Mouse
Protein Sequence	FTITAPKDLY VVEYGSNVTM ECRFPVEREL DLLALVVYWE KEDEQVIQFV AGEEDLKPQH SNFRGRASLP KDQLLKGNAA LQITDVKLQD AGVYCCIISY GGADYKRITL KVNAPYRKIN QRISVDPATS EHELICQAEG YPEAEVIWTN SDHQPVSGKR SVTTSRTEGM LLNVTSSLRV NATANDVFYC TFWRSQPGQN HTAELIIPEL PATHPPQNRT VDDIEGRMDE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Disclaimer	For research use only

Alternative Names

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B7H1; PDCD1L1; PDCD1LG1; PDL1; Programmed cell death 1 ligand 1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7 homolog 1; B7-H1; CD274

Human PD-L1 Protein, mFc-His tag [orb689384]

Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 52.3 kDa after removal of the signal peptide.
Source:	Mammalian

Sizes Available: 100 μg, 10 μg, 50 μg

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Mouse CD274 protein [orb391394]
Applications:
ELISA, MS, SDS-PAGE, WB
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Source:
Human cells
Sizes Available: 10 μg, 50 μg, 500 μg
Item 1 of 1
Recombinant mouse PD-L1 protein, C-His (HEK293) [orb1808045]
Purity:
>90% as determined by SDS-PAGE
MW:
26.8 kDa
Sizes Available: 20 μg, 100 μg, 500 μg
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Recombinant mouse PD-L1 protein, C-hFc (HEK293) [orb1808046]
Purity:
>90% as determined by SDS-PAGE
MW:
51.5 kDa
Sizes Available: 20 μg, 100 μg, 500 μg

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Immobilized Recombinant Mouse CD274 Protein at 2 μg/ml (100 μl/well) can bind Anti-CD274 antibody. The ED50 of Anti-CD274 antibody is 0.008 μg/ml.

Quick Database Links

UniProt

Q9EP73

NCBI Gene ID

60533

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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