Mouse CD40 protein

SKU: orb707155

Description

Recombinant Mouse CD40 Protein is produced by mammalian expression system and the target gene encoding Leu20-Arg193 is expressed with a Fc tag at the C-terminus.

Research Area

Epigenetics, Immunology

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	Human cells
Reactivity	Mouse
Protein Sequence	LGQCVTCSDK QYLHDGQCCD LCQPGSRLTS HCTALEKTQC HPCDSGEFSA QWNREIRCHQ HRHCEPNQGL RVKKEGTAES DTVCTCKEGQ HCTSKDCEAC AQHTPCIPGF GVMEMATETT DTVCHPCPVG FFSNQSSLFE KCYPWTSCED KNLEVLQKGT SQTNVICGLK SRMRVDDIEG RMDEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSREEMTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGK
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Disclaimer	For research use only

Alternative Names

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TNFRSF5; Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40; CD40

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Quick Database Links

UniProt

P27512

NCBI Gene ID

21939

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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