Mouse CD40LG protein

SKU: orb707113

Description

Recombinant Mouse CD154 Protein is produced by mammalian expression system and the target gene encoding Met112-Leu260 is expressed with a 6His tag at the N-terminus.

Research Area

Epigenetics

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	Human cells
Reactivity	Mouse
Protein Sequence	GSGSHHHHHH IEGRGGGSGG GSGGGSMQRG DEDPQIAAHV VSEANSNAAS VLQWAKKGYY TMKSNLVMLE NGKQLTVKRE GLYYVYTQVT FCSNREPSSQ RPFIVGLWLK PSSGSERILL KAANTHSSSQ LCEQQSVHLG GVFELQAGAS VFVNVTEASQ VIHRVGFSSF GLLKL
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Disclaimer	For research use only

Alternative Names

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CD40L; TNFSF5; TRAP; CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD154

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Quick Database Links

UniProt

P27548

NCBI Gene ID

21947

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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