Mouse Complement C5 Protein, His Tag

SKU: orb653662

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Description

Mouse Complement C5, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Glu 1680 (Accession # P06684-1).

Research Area

Immunology & Inflammation

Images & Validation

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Application Notes

This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.5 kDa (β chain) and 114.6 kDa (α chain). The protein migrates as 66 kDa (β chain) and 100 kDa (α chain) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression System	HEK293
Target	Complement C5
Reactivity	Mouse
Tag	C-10×His
Molecular Weight	73.5 kDa (β chain) & 114.6 kDa (α chain)
Expression Region	Gln 19 - Leu 678 & Asn 679 - Glu 1680
Purity	95%
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated

Storage & Handling

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Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/Appearance	Powder
Buffer/Preservatives	PBS, pH 7.4
Disclaimer	For research use only

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Immobilized Mouse Complement C5, His Tag (Cat. No. orb653662) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.1-13 ng/mL (QC tested).

Quick Database Links

Gene Symbol

Complement C5

UniProt

P06684

UniProt Details

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No UniProt data available

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