Mouse CXCL15(Chemokine C-X-C-Motif Ligand 15) Microsample ELISA Kit

SKU: orb2997294

Description

Images & Validation

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Application Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CXCL15. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CXCL15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CXCL15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CXCL15 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	78.13-5000 pg/mL
Sensitivity	46.38 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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C-X-C motif chemokine 15;Chemokine (C-X-C motif) ligand 15;Cxcl15;CXL15;Lungkine;Scyb15;Small inducible cytokine B15;Small inducible cytokine subfamily B member 15;Small-inducible cytokine B15;weche

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UniProt

Q9WVL7

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