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Description
Images & Validation
−Item 1 of 2
| Tested Applications | FC, WB |
|---|---|
| Dilution Range | WB: 1:1000, FC: 1:25 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This Mouse Epcam antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 302-335 amino acids from the C-terminal region of mouse Epcam. Antigen Region: 302-335 aa. |
| Target | Epcam |
| Molecular Weight | 35019 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Epithelial cell adhesion molecule, Ep-CAM, Epithelial glycoprotein 314, EGP314, mEGP314, Protein 289A, Tumor-associated calcium signal transducer 1, CD326, Epcam, Tacstd1
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Flow cytometric analysis of HepG2 cells using (Mouse) Epcam Antibody (C-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
All lanes: Anti-Epcam Antibody (C-term) at 1:1000 dilution. Lane 1: mouse kidney lysates. Lane 2: mouse lung lysates. Lane 3: mouse testis lysates. Lane 4: MCF-7 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Quick Database Links
Gene Symbol
Epcam
UniProt
UniProt Details
− No UniProt data available
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Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
(Mouse) Epcam Antibody (C-term) (orb1788010)
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