Mouse FAS protein

SKU: orb707174

Description

Recombinant Mouse CD95 Protein is produced by mammalian expression system and the target gene encoding Gln22-Arg169 is expressed with a 6His tag at the C-terminus.

Research Area

Cell Biology

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	Human cells
Target	FAS
Reactivity	Mouse
Endotoxins	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Protein Sequence	QGTNSISESL KLRRRVRETD KNCSEGLYQG GPFCCQPCQP GKKKVEDCKM NGGTPTCAPC TEGKEYMDKN HYADKCRRCT LCDEEHGLEV ETNCTLTQNT KCKCKPDFYC DSPGCEHCVR CASCEHGTLE PCTATSNTNC RKQSPRNRHH HHHH

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of PBS, pH 7.4.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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APT1; FAS1; TNFRSF6; Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; CD95

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Quick Database Links

Gene Symbol

FAS

UniProt

P25446

NCBI Gene ID

14102

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Protein Handling and Storage Guide

Protein Handling Guide

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Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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