Mouse Glucagon Like Peptide 2 (GLP-2) ELISA Kit

SKU: orb1807731

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Description

Mouse Glucagon Like Peptide 2 (GLP-2) ELISA Kit, uses a competitive method for detection and can measure the content of GLP-2 in serum, plasma, tissue homogenates, and other biological samples.

Research Area

Metabolism Research

Images & Validation

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Application Notes

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse GLP-2. During the reaction, Mouse GLP-2 in the sample or standard competes with a fixed amount of Mouse GLP-2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse GLP-2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse GLP-2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Mouse
Sample Types	Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL
Assay Type	Competitive
Assay Time	1.5H
Range	78.13-5000pg/mL
Sensitivity	46.88 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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GLP2

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Mouse Glucagon Like Peptide 2 (GLP-2) ELISA Kit

Quick Database Links

Gene Symbol

GLP-2

UniProt

P55095

UniProt Details

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No UniProt data available

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