Mouse IgG (H&L) Antibody Peroxidase Conjugated

SKU: orb347619

Description

Mouse IgG (H&L) antibody (Peroxidase)

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:100,000 - 1:200,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
Reactivity	Mouse
Application Notes	Anti-Mouse IgG Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Key Properties

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Antibody Type	Secondary Antibody
Host	Sheep
Clonality	Polyclonal
Isotype	IgG
Immunogen	Anti-Mouse IgG [H&L] was produced by repeated immunization with Mouse IgG whole molecule in sheep.
Purity	Sheep Anti-Mouse IgG[H&L] Peroxidase Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum, Mouse IgG and Mouse Serum.
Conjugation	HRP

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Concentration	2.0 mg/mL
Disclaimer	For research use only

Alternative Names

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sheep anti-Mouse IgG heavy and light chain peroxidase conjugated Antibody, sheep anti-Mouse IgG HRP secondary Antibody, sh anti-ms IgG HRP antibody

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Mouse IgG (H&L) Antibody Peroxidase Conjugated

Western Blot of Peroxidase conjugated Sheep anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase sheep secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Mouse IgG. Other band(s): none.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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