Mouse Immunoglobulin G3 (IgG3) ELISA Kit

SKU: orb866936

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Description

Sandwich Mouse IgG3 ELISA Kit

Research Area

Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 200 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IgG3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IgG3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IgG3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	3.13-200 ug/mL
Sensitivity	1.48 ug/mL
Concentration	200 µg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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IGHG3 ELISA Kit, HDC ELISA Kit, Heavy chain disease protein ELISA Kit, Ig Gamma-3 Chain C Region ELISA Kit, Immunoglobulin Heavy Constant Gamma 3 ELISA Kit, G3m Marker ELISA Kit, Immunoglobulin Gm3 ELISA Kit

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Mouse Immunoglobulin G3 (IgG3) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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