Mouse Pregnancy Associated Plasma Protein A (PAPPA) ELISA Kit

SKU: orb775301

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pregnancy Associated Plasma Protein A(PAPPA).

Research Area

Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PAPPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PAPPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PAPPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PAPPA in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	78.13-5000 pg/mL
Sensitivity	29 pg/mL
Concentration	5000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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PAPP-A ELISA Kit, PAPA ELISA Kit, ASBABP2 ELISA Kit, DIPLA1 ELISA Kit, IGFBP-4ase ELISA Kit, PAPPA1 ELISA Kit, Pappalysin 1 ELISA Kit, Insulin-Like Growth Factor Binding Protein-4 Protease ELISA Kit, Differentially Placenta 1 Expressed Protein ELISA Kit

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Mouse Pregnancy Associated Plasma Protein A (PAPPA) ELISA Kit

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UniProt

Q8R4K8

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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