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Mouse/Rat TGF-beta 1
SKU: orb3002482
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Activity | ELISA: Immobilized Mouse/Rat TGF-beta 1(Cat#orb3002482) at 2μg/ml (100 μl/well) can bind Mouse TGFBR2-His(Cat#orb3002250).The ED50 of Mouse TGFBR2-His(Cat#orb3002250)is 2.5 ug/ml.. Cell Based Assay: Measured by its ability to inhibit IL-4-dependent proliferation of TF‑1 human erythroleukemic cells.The ED50 for this effect is 5-25 pg/ml. (QC verified) |
| Tag | Tag free |
| Molecular Weight | Predicted: 12.8 KDa. Observed: 13 KDa, reducing conditions |
| Expression Region | Ala279-Ser390 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 4mM HCl,4%Mannitol. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in 4mM HCl. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−TGF-beta-1; TGFB; TGF-b1; TGFB1; CEDLAP;latency-associated peptide; TGFbeta; TGF-beta 1 protein; transforming growth factor beta-1
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Mouse/Rat TGF-beta 1 (orb3002482)
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