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Multi-rAb CoraLite Plus 750-Goat Rabbit Recombinant Secondary Antibody (H+L)

SKU: orb2302682

Description

This Multi-rAb antibody targets Rabbit IgG (H+L) in FC and IF. It is higly specific to rabbit IgG and shows minimal reactivity for mouse, rat, human, pig and bovine IgGs.

Images & Validation

Tested ApplicationsFC, IF, WB
Dilution Range1:200-1:1000 IF and for FC, 1:5000-1:20,000 for western blotting
ReactivityMouse

Key Properties

Antibody TypeRecombinant Antibody
HostGoat
ClonalityRecombinant
PurityThe antibody was purified from culture media supernatant by immunoaffinity chromatography using Protein G beads.
ConjugationCoraLite® Plus 750

Storage & Handling

StorageStore at -20°C. Stable for one year after shipment.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 50% glycerol, 10 mg/mL rAlbumin, 0.1% Proclin-300, pH 7.4.
Concentration0.5 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Multi-rAb CoraLite Plus 750-Goat Rabbit Recombinant Secondary Antibody (H+L)

Various lysates were subjected to SDS-PAGE followed by western blot with Rabbit anti-Beta Tubulin recombinant antibody. Multi-rAb CoraLite Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) orb2302682 was used at 1:10000 for detection.

Multi-rAb CoraLite Plus 750-Goat Rabbit Recombinant Secondary Antibody (H+L)

Various lysates were subjected to SDS-PAGE followed by western blot with Rabbit anti-Beta Catenin recombinant antibody at dilution of 1:40000. Multi-rAb CoraLite Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) orb2302682 was used at 1:10000 for detection.

Multi-rAb CoraLite Plus 750-Goat Rabbit Recombinant Secondary Antibody (H+L)

Various lysates were subjected to SDS-PAGE followed by western blot with Rabbit anti-Beta Tubulin recombinant antibody at dilution of 1:20000. Multi-rAb CoraLite Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) orb2302682 was used at 1:10000 for detection.

Multi-rAb CoraLite Plus 750-Goat Rabbit Recombinant Secondary Antibody (H+L)

Immunofluorescent analysis of at dilution of 1:200 and Multi-rAb CoraLite Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302682), Beta Actin antibody ( Clone: 2D4H5).

Multi-rAb CoraLite Plus 750-Goat Rabbit Recombinant Secondary Antibody (H+L)

Immunofluorescent analysis of at dilution of 1:200 and Multi-rAb CoraLite Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302682), CL488-Phalloidin (green).

Multi-rAb CoraLite Plus 750-Goat Rabbit Recombinant Secondary Antibody (H+L)

1X10^6 MOLT4 were surface stained with 0.2 ug Anti-HLA class I rabbit polyclonal antibody and Rabbit IgG Isotype Control. Multi-rAb CoraLite Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) orb2302682 was used at 1:500 for detection.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
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IF
Immunofluorescence
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Multi-rAb CoraLite Plus 750-Goat Rabbit Recombinant Secondary Antibody (H+L) (orb2302682)

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Available Sizes

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200 μl
¥ 2,730.00
1 ml
¥ 4,290.00
DispatchUsually dispatched within 5-10 working days
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