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Multi-rAb CoraLite Plus 750-Goat Mouse Recombinant Secondary Antibody (H+L)
SKU: orb2302689
Description
Images & Validation
−Item 1 of 11
| Tested Applications | FC, IF, WB |
|---|---|
| Dilution Range | 1:200-1:1000 for IF and FC, 1:5000-1:20,000 for western blotting |
| Reactivity | Mouse |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Goat |
| Clonality | Recombinant |
| Purity | The antibody was purified from culture media supernatant by immunoaffinity chromatography using Protein G beads. |
| Conjugation | CoraLite® Plus 750 |
Storage & Handling
−| Storage | Store at -20°C. Stable for one year after shipment. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 50% glycerol, 10 mg/mL rAlbumin, 0.1% Proclin-300, pH 7.4. |
| Concentration | 0.5 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Multi-rAb CoraLite Plus 750-Goat Rabbit Recombinant Secondary Antibody (H+L) [orb2302682]
FC, IF, WB
Mouse
Goat
Recombinant
CoraLite® Plus 750
200 μl, 1 ml
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Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-Beta Tubulin monoclonal antibody (isotype IgG2a). Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) orb2302689 was used at 1:10000 for detection.
Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-ZNF174 monoclonal antibody (isotype IgG3). Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) orb2302689 was used at 1:10000 for detection.
Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-EIF3E monoclonal antibody (isotype IgG1) at dilution of 1:50000. Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) orb2302689 was used at 1:10000 for detection.
Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-Catalase monoclonal antibody (isotype IgG1) at dilution of 1:10000. Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) orb2302689 was used at 1:10000 for detection.
Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-Beta Tubulin monoclonal antibody (isotype IgG2a) at dilution of 1:100000. Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) orb2302689 was used at 1:10000 for detection.
Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-FAK monoclonal antibody (isotype IgG2c) at dilution of 1:5000. Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) orb2302689 was used at 1:10000 for detection.
Various lysates were subjected to SDS-PAGE followed by western blot with mouse anti-ZNF174 monoclonal antibody (isotype IgG3) at dilution of 1:2000. Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) orb2302689 was used at 1:10000 for detection.
Immunofluorescence of A431 cells: PFA-fixed cells were stained with anti-B23, followed by Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (orb2302689, 1:500, red), nuclei stained with DAPI (cyan). Confocal images were acquired with a 63x oil objective and post-processed.
Immunofluorescence of A431 cells: PFA-fixed cells were co-stained with mouse anti-B23 and rabbit anti-Lamin B1, followed by Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (orb2302689, 1:500, red) and Nano-Secondary srb2GCL555-1 (yellow). Confocal images were acquired with a 63x oil objective and post-processed. Device setting for orb2302689: Laser Ex max 764nm, filter Em max 775nm.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody ( Clone: 1E4C11 ) at dilution of 1:400 and Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (orb2302689), CL488-Phalloidin (green).
1X10^6 MOLT4 were surface stained with 0.2 ug Anti-Human CD8 (Clone: UCHT4) and Mouse IgG2a Isotype Control. Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) orb2302689 was used at 1:500 for detection.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
Multi-rAb CoraLite Plus 750-Goat Mouse Recombinant Secondary Antibody (H+L) (orb2302689)
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